Cells, Free Full-Text
Por um escritor misterioso
Descrição
Olive flounder (Paralichthys olivaceus) muscle satellite cells (OFMCs) were obtained by enzymatic primary cell isolation and the explant method. Enzymatic isolation yielded cells that reached 80% confluence within 8 days, compared to 15 days for the explant method. Optimal OFMC growth was observed in 20% fetal bovine serum at 28 °C with 0.8 mM CaCl2 and the basic fibroblast growth factor (BFGF) to enhance cell growth. OFMCs have become permanent cell lines through the spontaneous immortalization crisis at the 20th passage. Olive flounder skeletal muscle myoblasts were induced into a mitogen-poor medium containing 2% horse serum for differentiation; they fused to form multinucleate myotubes. The results indicated complete differentiation of myoblasts into myotubes; we also detected the expression of the myogenic regulatory factors myoD, myogenin, and desmin. Upregulation (Myogenin, desmin) and downregulation (MyoD) of muscle regulation factors confirmed the differentiation in OFMCs.
Nucleic acid biomarkers of immune response and cell and tissue damage in children with COVID-19 and MIS-C - ScienceDirect
Biology, Free Full-Text
Serial Number Alcohol 120 1.9 6 - Colaboratory
Frontiers Combining Cell-Free Protein Synthesis and NMR Into a Tool to Study Capsid Assembly Modulation
Electrochemical characterization of anode‐free K‐metal full‐cells. a)
A) Cell-free expression of sfGFP fused to a variety of N-and
Ds Kumar Strömungsmechanik Pdf Kostenloser Download - Colaboratory
Rapid cell-free characterization of multi-subunit CRISPR effectors and transposons - ScienceDirect
Sequencing of Circulating Cell-free DNA during Pregnancy
The cell-free system: A new apparatus for affordable, sensitive, and portable healthcare - ScienceDirect
Cells, Free Full-Text
IJMS, Free Full-Text
de
por adulto (o preço varia de acordo com o tamanho do grupo)
